Researchers at North Carolina State University have developed a new approach for manipulating the behavior of cells on semiconductor materials, using light to alter the conductivity of the material itself.
“There’s a great deal of interest in being able to control cell behavior in relation to semiconductors – that’s the underlying idea behind bioelectronics,” says Albena Ivanisevic, a professor of materials science and engineering at NC State and corresponding author of a paper on the work. “Our work here effectively adds another tool to the toolbox for the development of new bioelectronic devices.”
The new approach makes use of a phenomenon called persistent photoconductivity. Materials that exhibit persistent photoconductivity become much more conductive when you shine a light on them. When the light is removed, it takes the material a long time to return to its original conductivity.
When conductivity is elevated, the charge at the surface of the material increases. And that increased surface charge can be used to direct cells to adhere to the surface.
“This is only one way to control the adhesion of cells to the surface of a material,” Ivanisevic says. “But it can be used in conjunction with others, such as engineering the roughness of the material’s surface or chemically modifying the material.”
For this study, the researchers demonstrated that all three characteristics can be used together, working with a gallium nitride substrate and PC12 cells – a line of model cells used widely in bioelectronics testing.
The researchers tested two groups of gallium nitride substrates that were identical, except that one group was exposed to UV light – triggering its persistent photoconductivity properties – while the second group was not.
“There was a clear, quantitative difference between the two groups – more cells adhered to the materials that had been exposed to light,” Ivanisevic says.
“This is a proof-of-concept paper,” Ivanisevic says. “We now need to explore how to engineer the topography and thickness of the semiconductor material in order to influence the persistent photoconductivity and roughness of the material. Ultimately, we want to provide better control of cell adhesion and behavior.”
The paper, “Persistent Photoconductivity, Nanoscale Topography and Chemical Functionalization Can Collectively Influence the Behavior of PC12 Cells on Wide Band Gap Semiconductor Surfaces,” is published in the journal Small. Lead author of the paper is Patrick Snyder, a Ph.D. student in Ivanisevic’s lab. The paper was co-authored by Ronny Kirste of Adroit Materials, and Ramon Collazo, an assistant professor of materials science and engineering at NC State.
The work was done with support from the U.S. Army Research Office, under grant number W911NF-15-1-0375, and the National Science Foundation, under grant number DMR-1312582.
Note to Editors: The study abstract follows.
“Persistent Photoconductivity, Nanoscale Topography and Chemical Functionalization Can Collectively Influence the Behavior of PC12 Cells on Wide Band Gap Semiconductor Surfaces”
Authors: Patrick J. Snyder, Ramon Callozo and Albena Ivanisevic, North Carolina State University; Ronny Kirste, Adroit Materials
Published: April 28, Small
Abstract: Wide bandgap semiconductors such as gallium nitride (GaN) exhibit persistent photoconductivity properties. This work incorporates this asset into the fabrication of a unique biointerface. Templates with lithographically defined regions with controlled roughness are generated during the semiconductor growth process. Template surface functional groups are varied using a benchtop surface functionalization procedure. The conductivity of the template is altered by exposure to UV light and the behavior of PC12 cells is mapped under different substrate conductivity. The pattern size and roughness are combined with surface chemistry to change the adhesion of PC12 cells when the GaN is made more conductive after UV light exposure. Furthermore, during neurite outgrowth, surface chemistry and initial conductivity difference are used to facilitate the extension to smoother areas on the GaN surface. These results can be utilized for unique bioelectronics interfaces to probe and control cellular behavior.